Taxonomía filogenética y molecular de Trypanosoma spp con marcadores de ADN 18S ITS y Catepsina L

Abstract

Diseases caused by Trypanosoma spp are one of the causes of death in tropical countries, these parasites being transmitted mainly by blood-sucking insects. The Trypanosoma genus has become a zoonotic agent that affects domestic and wild animals, as well as humans tangentially. Ecuador is a country that is located in a region with the presence of tropical and zoonotic diseases, there is not enough data to show a more complete picture of these diseases. The lack of a "Gold Standard" for the identification of different trypanosomes has been a problem for obtaining data, which is why different molecular markers have been developed that are directed towards different regions of the genome of these parasites. Among these markers are the transcribed spacer (ITS), cathepsin L-like protein (CatL) and the small subunit 18SrRNA and together create a phylogenetic matrix for the identification of Trypanosoma spp. The MEGA 11 program was used for the alignment of the obtained sequences, a phylogenetic tree was constructed from each marker with the Maximum Parsimony method. As a result, several parsimonious trees were obtained from each of the molecular markers, the one with the highest relationship and with a high bootstrap was chosen, thus being able to validate the matrix created for the phylogenetic differentiation of each trypanosome