Aislamiento, identificación y caracterización de microorganismos de interés industrial y ambiental para crear un cepario de la Facultad de Ingeniería y Ciencias Aplicadas (FICA) de la Universidad Internacional SEK

Abstract

Biotechnology is gaining an importance in improving industrial processes to meet human needs. Technology applications are used to obtain products and/or services, or modify existing traditional processes. UISEK doesn’t have isolated microorganisms for laboratory practices or thesis work. In this context, the purpose of this study is to isolate, identify and characterize different types of bacteria of industrial and environmental interest, for the generation of a cepareum at the FICA of UISEK. The results obtained with phenotypic identification techniques were compared: Morphology (Gram staining), biochemical tests and proteomics by MALDI – TOF, obtaining positive identifications from 8 different bacteria. In addition, and since the objective was to generate a ceparium and keep it frozen for later teaching or research uses, the best way to preserve these microorganisms was evaluated by testing two methods of cryopreservation with glycerol: 10% and 20%. The differences between the two methods were not very noticeable although there was a slight general tendency to grow better after the cytopreservation at 10%.