Sero-epidemiología e identificación de Brucella SPP. con técnicas moleculares en ganado bovino en la provincia del Carchi

Abstract

Brucellosis is a zoonotic disease caused by Brucella spp. bacteria, of relevance for generating economic losses in the livestock sector and for being transmitted to humans. In bovines it is characterized by giving abortions and in humans it appears in the great majority as systematic diseases. In Ecuador the prevalence of brucellosis is 17% and in the Province of Carchi 10%, according to data from 40 years ago to the current date of the presentation of this work, therefore, it is necessary to carry out investigations of the current epidemiology and identification of Brucella spp. circulating in the province of Carchi. The aim of the present study will be to determine the seroprevalence and molecular identification associated with bovine brucellosis in Carchi province. A total of 384 blood samples will be collected from cows. Initially, all samples will be tested for anti-Brucella antibodies using the Rose Bengal (RB) plaque test. RB positive serum samples will be confirmed by competitive ELISA assay (cELISA). Subsequently, a molecular identification of the seropositive animals will be carried out with cELISA, from uterus tissues, retromammary lymph nodes and udders by means of the AMOS quantitative polymerase chain reaction (PCR) assay. To obtain the genetic material used in AMOS PCR, it will be done using a commercial kit and an in-house method. The results will be evaluated through a descriptive analysis and kappa index (k). With this research will be expected to obtain information regarding the sero-epidemiology of bovine brucellosis and the species of the causal agent, which in the future will be used for the control and eradication of brucellosis in the province of Carchi.